22 Apr 2017 (ABI5) in Arabidopsis. The AGL21-overexpressing plants were hypersensitive to ABA, salt, and osmotic stresses during seed germination and

The relative hypocotyl lengths of Col-0, abi5-1, and abi5/ZFP3ox seedlings were similar at different fluence rates of red light. In contrast to the Col-0 background, ZFP3 overexpression could not reduce relative hypocotyl lengths in the abi5-1 mutant background upon red light illumination .

The endogenous ABI5 phosphorylation and inhibition of germination could be recapitulated by the addition of a SnRK2 protein kinase to the ABI5 overexpression line. In sleepyl mutant seeds, RGL2 overaccumulates; germination of these seeds can occur under conditions that produce low ABI5 expression. These data Several ABI5/ABF binding proteins (AFPs) inhibit ABA response, resulting in extreme ABA resistance in transgenic Arabidopsis overexpression lines, but their mechanism of action has remained obscure. By analogy to the related Novel Interactor of JAZ (NINJA) protein, it was suggested that the AFPs interact with the co-repressor TOPLESS to inhibit ABA-regulated gene expression.

Abi5 overexpression

Furthermore, we showed that ABI5 directly binds to the CAT1promoter and activates CAT1expression. Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germi- nation. Se hela listan på frontiersin.org Overexpression of ABI5 Confers ABA Sensitivity to hy5 and Inhibits Hypocotyl Elongation. If ABI5 is a direct target of HY5, one would expect that overexpression of ABI5 may be able to rescue some of the hy5 mutant phenotypes such as its reduced sensitivity to ABA. The ABI5 overexpression lines were generated in both Col-0 and hy5 background 2020-04-11 · Functional analysis indicated that overexpression of GmSUMO2 gene in soybean hairy roots accentuated the sensitivity to exogenous ABA. Furthermore, the expression levels of ABI3, ABI5, SnRK1.1 and SnRK1.2 were differentially regulated by GmSUMO2 in transgenic soybean hairy roots. Abscisic acid‐insensitive 5 (ABI5) is an essential and conserved plant basic leucine zipper transcription factor whose level controls seed germination and postgerminative development.

Loss-of-function in ABI5 (abi5) promotes juvenile-to-adult transition, whereas overexpression of ABI5 delays this transition in short days. Genetic analyses indicated that the effect of mir159ab on vegetative phase change is ABI5 dependent.

Overexpression of MdABI5 promoted ABA-induced anthocyanin biosynthesis, which was partially dependent on MdbHLH3. ABI5 Binding Proteins (AFPs) are a family of negative regulators of bZIP transcription factors of the AREB/ABF family, which promote ABA responses. AFP2 interacts with Snf1-Related protein Kinase 1 (SnRK1), which belongs to a highly conserved heterotrimeric kinase complex that is activated to re-establish energy homeostasis following stress.

ABI5 affects reactive oxygen species (ROS) homeostasis by affecting CATALASEexpression and cata- lase activity. Furthermore, we showed that ABI5 directly binds to the CAT1promoter and activates CAT1expression. Genetic evidence supports the idea that CAT1 functions downstream of ABI5 in ROS signaling during seed germi- nation.

Thus, we compared the transcription of FLC in Col, afp2, and AFP2-ox under LD conditions.

Analyses of transcript accumulation in abi5 mutants suggest that, similar to ABI3, ABI5 has both activator and repressor functions that may have either synergistic or antagonistic effects on gene expression, depending on the target gene. Indeed, stomatal aperture was significantly reduced by ABI5 overexpression in the absence or presence of ABA under monochromatic light conditions. Overall, we present a regulatory loop in which two light signalling proteins repress ABA signalling to sustain gas exchange when plants experience periodic drought. However, overexpression of ABI5 was not sufficient to repress germination, as ABI5 activity requires phosphorylation. The endogenous ABI5 phosphorylation and inhibition of germination could be recapitulated by the addition of a SnRK2 protein kinase to the ABI5 overexpression line. ABI5-BINDING PROTEIN2 (AFP2) negatively regulates the abscisic acid signal by accelerating ABI5 degradation during seed germination in Arabidopsis (Arabidopsis thaliana).
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Genetic analyses indicated that the effect of mir159ab on vegetative phase change is ABI5 dependent. ABI5 is necessary, but not sufficient, to maintain germinated embryos in a quiescent state, abscisic acid also being required for maintaining the quiescent state. ABI5 production is enhanced by stress including high salt and drought. This protects plants from drought. 2008-03-18 However, overexpression of ABI5 was not sufficient to repress germination, as ABI5 activity requires phosphorylation.

This protects plants from drought.
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(B) Phenotypes of abi5-1, ABI5 overexpression lines (OE) and WT seeds sown on plates containing DMSO, ABA (1 μM), and AZD (1 μM) for 14 days. Supplementary Figure 3 | GUS staining of ABI5-GUS OE12 transgenic line. (A) GUS staining of leaves of 7-day-old ABI5-GUS OE12 plants treated with DMSO, ABA (50 μM), and AZD (2 μM) for 48 h.

Our study suggests that FHY3 and FAR1 are positive regulators of ABA signaling.